Digynic triploidy in rabbit blastocysts after delayed insemination

Digynic triploidy: utility and challenges of noninvasive prenatal testing

Low fraction fetal DNA in noninvasive prenatal testing in the context of fetal growth restriction and multiple congenital anomalies should alert medical professionals to the possibility of digynic triploidy. Single-nucleotide polymorphism microarray can detect the parental origin of triploidy and explain its mechanism.

Comparative evaluation of pregnancy rates in Dutch laboratory rabbit after insemination and natural breeding

Effect of copper on isolated rabbit blastocysts.

The effect of copper on the electrical membrane properties of the isolated-perfused 6-day rabbit blastocyst was studied to understand changes in the intrauterine environment caused by the copper IUD. Blastocysts were perfused in an environmental chamber containing Krebs-Ringer bicarbonate with 1 mg bovine serum albumin/ml. Electrical measurements made included short-circuit current (SCC) (the...

Cytogenetic analysis of giant oocytes and zygotes to assess their relevance for the development of digynic triploidy.

BACKGROUND In order to assess the role of binucleate giant oocytes for generating digynic triploidy, we studied their frequency, maturation patterns and chromosomal complements at metaphase II (MII) or after fertilization. METHODS Uncleaved, giant zygotes were incubated with podophyllotoxin and vinblastine, treated with hypotonic solution and fixed by a gradual fixation method. Giant MII oocy...

Cyclic AMP and cyclic GMP in rabbit blastocysts.

Concentrations of both nucleotides were significantly higher in Day-6 than in Day-5 blastocysts but the ratio of cAMP to cGMP changed from 0.5 to 1.5.